유체에 의해 유발된 전단력이 치은 섬유아세포 유전자 발현 변화에 미치는 영향에 관한 연구
Gene expression after the application of the fluid-induced shear stress on the gingival fibroblast
정미향, 최재용, 채창훈, 김성곤, 남동석,
소속 상세정보
정미향 ( Jeong Mi-Hyang ) - 서울대학교 치과대학 교정학교실
최재용 ( Choi Jae-Yong ) - 경북대학교 의과대학 생화학교실
채창훈 ( Chae Chang-Hoon ) - 한림대학교 의과대학 구강악안면외과학교실
김성곤 ( Kim Seong-Gon ) - 한림대학교 의과대학 구강악안면외과학교실
남동석 ( Nam Dong-Seok ) - 서울대학교 치과대학 교정학교실
KMID : 0360120050270050424
Abstract
The oral cavity is humid environment mainly due to the continuous salivary flow. The reaction of oral mucosa to fluid flow is important for homeostasis and pathogenesis. The objective of this study is the screening the change of gene expression after the application of fluid induced shear stress (FISS) on the gingival fibroblast using cDNA microarray assay. The immortalized human gingival fibroblasts were grown and FISS was applied using a cone viscometer at a rotational velocity of 40 rpm, respectively for periods of 2 and 4 hours. The synthesis of cDNA was done from the extracted total RNA and cDNA microarray assay was done subsequently. The genes that showed over 1.6 in the Cy3/Cy5 or the Cy5/Cy3 value were regarded as genes influenced significantly by the FISS application ion (/M/>0.7). The " RUNX-1" was increased its expression in 2 hours group and " RUN and SH3 domain containing 1" was increased its expression in 4 hours group. The "CC020415", "cyclin L1", "interferon regulatory factor1", "early growth response 1", "immediate early response 2", and "immediate early response 3" genes were increased their expression in 2 and 4 hours after FISS application. In conclusion, we could find many genes that were probably related to the FISS application. Interestingly, most of them were placed in similar molecular pathways and these findings improve the reliability of chip data and usefulness in overall screening. From this experiment, we could find many items for further study and it will make improvement in the understanding of intracellular events in response to FISS.
키워드
Gingival fibroblast;cDNA microarray;Fluid induced shear stress
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